Journal: Journal of Pharmacognosy and Phytochemistry
Author: Mohamed Elgindi, Nahla Ayoub, Rola Milad*, Reham Mekky
Abstract: Cuphea hyssopifolia Kunth (Family Lythraceae) is a herb, newly cultivated in Egypt. Its richness in phenolics had led to test for its antioxidant and cytotoxic activities and isolation of its componenets. Also it urges us to check for its genotyping. Isolation of some phenolics was done using usual chromatographic techniques. The genetic fingerprinting of the aerial parts of C. hyssopifolia Kunth was determined using RAPD-PCR (Random Amplified Polymorphic DNA) technique which has been widely used in plants for the construction of genetic maps using 10 primers. The antioxidant activity of the extract was measured using the stable free radical DPPH assay while its cytoctoxic property was tested using different cell lines viz MCF7 (breast carcinoma cell line), HEP2 (larynx carcinoma cell line), HCT116 (colon carcinoma cell line) and HEPG2 (liver carcinoma cell line) adopting SRB method.Several compounds had been isolated from the aqueous methanolic extract of the aerial parts of C. hyssopifolia Kunth namely valoneic acid dilactone (1), 1,3−O−digalloyl-4-,6-hexahydroxydiphenoyl- β-D−4C1-glucopyarnose (2), gallic acid (3), genistein-7-O-β-D-4C1-glucopyranoside (4), myricetin−3 − O− β-D−4C1-glucopyarnoside (5), 3, 4, 5-trimethoxy benzoic acid (6), vanilic acid (7) and quercetin (8). Primer OPP-01 was the best sequence for dominating C. hyssopifolia Kunth cultivated in Egypt. The antioxidant activity of the extract showed an inhibition 95.5% compared to that of ascorbic acid 98.35%. The extract showed moderate cytotoxic activity with IC50 ranging from 73.4 to 92.5 ug/ml for the tested cell lines. The study suggests that C. hyssopifolia Kunth may be the potential rich source of natural antioxidant.
