Antimicrobial Potential Of Azadirachta Indica Against Pathogenic Bacteria And Fungi

Journal: Journal of Pharmacognosy and Phytochemistry

Author: Mohammad Asif*

Abstract: Drugs from natural sources are used for treating various diseases since the ancient times. From the literature it is clear that various type of pharmacological and biological activities are associated with Azadirachta indica. The leave oil of A. indica is known to have good antimicrobial potential. The oil of A. indica leaves, was tested against the different infectious microorganisms (Gram positive bacteria and Gram-negative bacteria), such as bacterial strains; S. aureus, E. coli, B. cerus, P. vulgaris, S. typhi, K. pneumonae, S. dysenterae and Fungal strains; F. oxysporum, A. flavus, A. fumigates, A. niger, C. albicans, Cladosporium sp., M. canis, M. gypseum, T. rubrum, T. mentagrophytes, P. notatum and P. citrinum etc. The results showed that level of antimicrobial activities of the A. indica oil depends on both the protein and carbohydrate contents. Generally, the high level of protein and carbohydrate contents of extract had better antimicrobial activities.

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Antioxidant and Cytotoxic Activities of Cuphea hyssopifolia Kunth (Lythraceae) Cultivated in Egypt

Journal: Journal of Pharmacognosy and Phytochemistry

Author: Mohamed Elgindi, Nahla Ayoub, Rola Milad*, Reham Mekky 

Abstract: Cuphea hyssopifolia Kunth (Family Lythraceae) is a herb, newly cultivated in Egypt. Its richness in phenolics had led to test for its antioxidant and cytotoxic activities and isolation of its componenets. Also it urges us to check for its genotyping. Isolation of some phenolics was done using usual chromatographic techniques. The genetic fingerprinting of the aerial parts of C. hyssopifolia Kunth was determined using RAPD-PCR (Random Amplified Polymorphic DNA) technique which has been widely used in plants for the construction of genetic maps using 10 primers. The antioxidant activity of the extract was measured using the stable free radical DPPH assay while its cytoctoxic property was tested using different cell lines viz MCF7 (breast carcinoma cell line), HEP2 (larynx carcinoma cell line), HCT116 (colon carcinoma cell line) and HEPG2 (liver carcinoma cell line) adopting SRB method.Several compounds had been isolated from the aqueous methanolic extract of the aerial parts of C. hyssopifolia Kunth namely valoneic acid dilactone (1), 1,3−O−digalloyl-4-,6-hexahydroxydiphenoyl- β-D−4C1-glucopyarnose (2), gallic acid (3), genistein-7-O-β-D-4C1-glucopyranoside (4), myricetin−3 − O− β-D−4C1-glucopyarnoside (5), 3, 4, 5-trimethoxy benzoic acid (6), vanilic acid (7) and quercetin (8). Primer OPP-01 was the best sequence for dominating C. hyssopifolia  Kunth cultivated in Egypt. The antioxidant activity of the extract showed an inhibition 95.5% compared to that of ascorbic acid 98.35%. The extract showed moderate cytotoxic activity with IC50 ranging from 73.4 to 92.5 ug/ml for the tested cell lines. The study suggests that C. hyssopifolia Kunth may be the potential rich source of natural antioxidant.

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An Overview on Traditional Medicinal Plants as Aphrodisiac Agent

Journal: Journal of Pharmacognosy and Phytochemistry

Author: Ramandeep Singh*, Sarabjeet Singh, G. Jeyabalan , Ashraf Ali

Abstract: This paper presents a review of plants identified from various ethno botanical surveys and folklore medicinal survey with aphrodisiac activity. An aphrodisiac is defined as an agent that arouses sexual desire.  Erectile dysfunction (ED) or male impotence is defined as the inability of a man to achieve and maintain an erection sufficient for mutually satisfactory intercourse with his partner. Sexual health and function are important determinants of quality of life. To overcome the problem of Male sexual (or) erectile dysfunction various natural aphrodisiac plants potentials are preferred. This review discuss about aphrodisiac potential of plants, its botanical name, Common name, family, part used and references, which are helpful for researcher to development new herbal aphrodisiac formulations.

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In Vitro Anthelmintic Activity of Lagenaria Siceraria Leaves in Indian Adult Earthworm

Journal: Journal of Pharmacognosy and Phytochemistry

Author: Amit Kumar*, Sangh Partap

Abstract: Different extracts of Lagenaria siceraria were taken for anthelmintic activity against Indian earthworm Pheritima posthuma. Two concentrations (50 and 100 mg/ml) of various extracts were tested and results were expressed in terms of time for paralysis and time for death of worms. Albendazole (20 mg/ml) was used as reference standard and carboxy methyl cellulose (0.5%) as a control group. Dose dependent activity was observed in the plant extracts but methanolic extract exhibited more activity as compared to others. The anthelmintic activity of  Lagenaria siceraria leaves extract has therefore been demonstrated for the first time.

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